First Aid for the USMLE Step 1 Quiz - Gradiuz

First Aid for the USMLE Step 1 Quiz

Quiz generated from First Aid for the USMLE Step 1

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Question 1

Which blotting technique is used to detect RNA?

  • Northern blot
  • Southern blot
  • Western blot
  • Eastern blot
Question 2

Which type of molecule is used as a probe in a Western blot?

  • Antibodies
  • DNA
  • RNA
  • Lipids
Question 3

What is the correct order of the main steps in PCR?

  • Denature DNA, anneal primers, elongate with heat-stable DNA polymerase
  • Anneal primers, denature DNA, elongate with heat-stable DNA polymerase
  • Elongate with heat-stable DNA polymerase, denature DNA, anneal primers
  • Denature DNA, elongate with heat-stable DNA polymerase, anneal primers
Question 4

Which cells are permanent cells that remain in G0?

  • Neurons, cardiac muscle cells, and red blood cells
  • Hepatocytes and lymphocytes
  • Bone marrow, gut, and skin cells
  • Goblet cells and plasma cells
Question 5

Which organelle is the primary site of steroid synthesis and detoxification of drugs and poisons?

  • Smooth endoplasmic reticulum
  • Rough endoplasmic reticulum
  • Golgi apparatus
  • Lysosome
Question 6

N-linked oligosaccharide addition occurs in which organelle?

Fill in the blank

Question 7

The Golgi apparatus adds mannose-6-phosphate to proteins to target them to lysosomes.

  • True
  • False
Question 8

What is the molecular defect in I-cell disease?

  • Failure to add mannose-6-phosphate in the Golgi
  • Defective microtubule polymerization
  • Failure of clathrin-mediated endocytosis
  • Inability to synthesize lysosomal enzymes
Question 9

COPII-coated vesicles mediate which transport step?

  • Anterograde transport from the rough endoplasmic reticulum to the cis-Golgi
  • Retrograde transport from the Golgi to the ER
  • Transport from the trans-Golgi to lysosomes
  • Transport from the plasma membrane to endosomes
Question 10

Chediak-Higashi syndrome results from a microtubule polymerization defect that decreases phagosome-lysosome fusion.

  • True
  • False
Question 11

Which organelle assembles proteoglycans from core proteins and sulfates sugars in proteoglycans?

  • Golgi apparatus
  • Rough endoplasmic reticulum
  • Smooth endoplasmic reticulum
  • Lysosome
Question 12

Which anti-breast cancer drug acts on microtubules?

  • Taxol
  • Tamoxifen
  • Methotrexate
  • Trastuzumab
Question 13

Which antifungal drug acts on microtubules?

  • Griseofulvin
  • Colchicine
  • Vincristine
  • Mebendazole
Question 14

Which of the following is an anticancer agent that acts on microtubules?

  • Vincristine
  • Griseofulvin
  • Thiabendazole
  • Colchicine
Question 15

Kinesin mediates anterograde transport, moving cargo from the minus end toward the plus end of the microtubule.

  • True
  • False
Question 16

Which drug used to treat gout acts on microtubules?

  • Colchicine
  • Griseofulvin
  • Vincristine
  • Mebendazole
Question 17

Dynein carries out which direction of transport along a microtubule?

  • Retrograde, from the plus end toward the minus end
  • Anterograde, from the minus end toward the plus end
  • Only along the central pair of microtubules
  • It does not move along microtubules
Question 18

Dynein moves cargo from the plus end toward the minus end of a microtubule.

  • True
  • False
Question 19

A dynein arm defect causing male and female infertility, bronchiectasis, and recurrent sinusitis is characteristic of which syndrome?

  • Kartagener syndrome
  • Marfan syndrome
  • Alport syndrome
  • Ehlers-Danlos syndrome
Question 20

Each alpha- and beta-tubulin dimer in a microtubule binds how many GTP molecules?

Fill in the blank

Question 21

Which statement best describes microtubule growth dynamics?

  • They grow slowly and collapse quickly
  • They grow quickly and collapse slowly
  • They grow and collapse at the same rate
  • They neither grow nor collapse once formed
Question 22

Which drug is an antihelminthic that acts on microtubules?

  • Mebendazole
  • Griseofulvin
  • Colchicine
  • Vincristine
Question 23

Collagen is the most abundant protein in humans.

  • True
  • False
Question 24

Collagen contains a repeating amino acid motif; identify it (written as three single-letter amino acid symbols separated by hyphens).

Fill in the blank

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